Study on Genetic Transformation of Hevea Brasiliensis

Title Study on Genetic Transformation of Hevea Brasiliensis
Abstract

Hevea brasiliensis is one of the important economical trees. For its characteristics of long producing-latex time and laticifer system, IT may be developed for the high-efficient and low-cost bioreactors, which has the large potential for commercial exploitation. Establishment of stable and high-efficiency genetic transformation system is the key step to develop the rubber tree into the bioreactor.Employing the anthers of Hevea Brasiliensis Haiken 2 、 Reyan88-13、 Reyan7-33-97 as explants, we investigated some factors involved in induction of callus and genetic transformation, and attempted to improve the efficiency of genetic transformation by employing the embryogenesis-related genes. The preliminary results are as follows.(1) Employing the anthers of Hevea Brasiliensis Haiken 2、 Reyan88-13、 Reyan7-33-97 as explants to induce the callus, the optimal temperature is 26°C in the daytime and 25 °C in the nighttime, and the concentration of ABA is 0.25mg/L in media.(2) According to brown degree of callus infected by Agrobacterium tumefaciens, the callus, about 25 days after inoculatation , was selected as explants for transformation and concentration of superpure sugrose was 3g/L in medium. We also found that the anther callus is insensitive to Kan and Kan was not suitable for screening the H.brasiliensis callus transformats.(3) PEI1, the gene encoding embryogenesis-related gene, was cloned by polymerase chain reaction from Arabidopsis and the plant expression vector pBIP with 35S::PEI1 was constructed.. The plant expression vectors, pBIP with 35S::PEIK pBIN with UBI::BnBBMl and pBI121, were respectively introduced into Agrobacterium tumefaciens strain LBA4404 and EHA105.(4) Some transformatied plants were obtained by Kan-resistance screening. A UBI::BnBBMl transgenic plant was respectively confirmed by histochemistry and PCR analysis. We could not get the 35S::PEI1 transgenic plants. The results implied that the efficiency of H.brasiliensis genetic transformation could not be obviously influenced by BnBBMl and PEIl genes.

Category Forestry
Keywords embryogenesis-related genes, Genetic transformation, Hevea brasiliensis,
FileType PDF
Pages 153
Price US$48.00
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